Blog
Chemistry workflows, cheminformatics insights, and updates from the ChemStitch team.
Preparing pH Buffers: Henderson-Hasselbalch in Practice
How to make buffer solutions using Henderson-Hasselbalch: worked acetate buffer example, buffer system selection by pH range, temperature corrections for Tris and HEPES, ionic strength effects, and common bench preparation mistakes.
Calculating Percent Yield: Why Your Reaction Gave Less Than Expected
How to calculate percent yield in organic synthesis: the formula, a worked amide coupling example with purity corrections, cumulative multi-step yield, loss troubleshooting, and reaction type benchmarks.
Limiting Reagent Calculations for Organic Synthesis: Scale-Up Without Wasted Material
How organic chemists calculate limiting reagents using equivalents: worked amide coupling example, purity corrections, scale-up considerations, and multi-step cumulative yield.
Serial Dilution for Assay Standards: Getting the Math Right Every Time
How to design and execute serial dilutions for standard curves: dilution factor selection, worked ELISA example, error propagation, mixing discipline, and curve quality checks.
Molarity Calculations in the Lab: From Powder to Working Stock
Step-by-step guide to calculating molarity from molecular weight, with worked examples, salt form corrections, purity adjustments, and common bench mistakes to avoid.